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Biodosimetry for persons exposed to alpha radiation: dependence on malignity (exp. work).
KŘÍŽOVÁ, Marcela
In the thesis I focus on evaluation of the effect of familiar occurrence of cancer on presence and number of micro-nuclei in blood preparations with particular groups of persons. One of the biodosimetry methods, test for presence, number and kind of micro-nuclei, was used. I was not investigating the dosages as such and connection of the number of micro-nuclei with the dose, however, I followed up the effect of potentially influencing side factor on presence and number of micro-nuclei, which is the familiar occurrence of tumour. As a lot of information about ionizing radiation, its sources, radioactivity, radio-biologically important quantities, mechanisms of effects, molecular and cellular changes and biodosimetric methods as such falls under the topic, the theoretical part of diploma project is dedicated to such information. In the practical part, evaluation of the number and kind of micro-nuclei with set groups of persons is carried out. These groups of persons are particularly exposed to ? radiation. They are employees of spa Lázně Jáchymov, former miners from already closed uranium mines in Příbram and current miners from uranium mines GEAM o. z. Dolní Rožínka. These selected persons are divided according to their workplaces into two groups, i.e. a group of persons without cancer occurrence in the family and a group of persons with occurrence of cancer in their families. The numbers of bi-nuclei cells without micro-nuclei and with micro-nuclei and the kinds of micro-nuclei were determined with all these persons using fluorescence microscope. Expression and comparison of percentages of bi-nuclei cells with micro-nuclei to total number of all bi-nuclei followed and then expression and comparison of proportion of micro-nuclei without centromeres to the number of micro-nuclei of both kinds (with centromeres and without centromeres) with all persons and groups were carried out. Based on the results gained from statistical assessment, it was found that differences between percentages of the two main compared groups of persons did not show higher genomic instability (statistically significant difference) in persons with familiar occurrence of cancer than in persons without cancer in the family. But by comparing the shares of micronuclei without centromeres was proved statistically significant difference between these main groups.
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